RNA

Part:BBa_J100085:Experience

Designed by: Caroline Vrana   Group: Campbell M Lab   (2012-07-13)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J100085

This sequence is missing one nucleotide (T) from the GFP target spacer (part E0040). This nucleotide is number 683 in the E0040 sequence, and the T should be between 181 and 182 in part J100085 sequence. This caused the system to not destroy the plasmid containing GFP as it should. CRISPR short.jpg

  • CRISPR and GFP should have no green fluorescence

User Reviews

UNIQ2f8849b3e7a1bf50-partinfo-00000000-QINU UNIQ2f8849b3e7a1bf50-partinfo-00000001-QINU